Stoichiometry of Site-Specific Protein Phosphorylation Estimated with Phosphopeptide-Specific Antibodies
نویسندگان
چکیده
منابع مشابه
Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
In recent years, antibodies specific for phosphopeptides have become a major tool for assessing the phosphorylation of specific serine, threonine, and tyrosine sites on proteins (1,2). Typically, a phosphopeptide-specific antibody is used for the qualitative detection of phosphorylation at a site through immunoblotting the protein of interest. Here we describe a relatively simple method by whic...
متن کاملCell cycle-dependent phosphorylation of centrosomes: localization of phosphopeptide specific antibodies to the centrosome.
The microtubule nucleation capacity of the centrosome increases dramatically as cells progress from interphase into mitosis. The increase in nucleation capacity of the centrosome correlates with the cell cycle-dependent localization of the mitotic protein monoclonal-2 (MPM-2) phosphoepitope-specific antibody to the mitotic centrosome. Therefore, the phosphorylation state of centrosomal componen...
متن کاملMammal-specific, ERK-dependent, caldesmon phosphorylation in smooth muscle. Quantitation using novel anti-phosphopeptide antibodies.
Extracellular signal-regulated kinases (ERKs) phosphorylate the high molecular mass isoform of the actin-binding protein caldesmon (h-CaD) at two sites (Ser(759) and Ser(789)) during smooth muscle stimulation. To investigate the role of phosphorylation at these sites, antibodies were generated against phosphopeptides analogous to the sequences around Ser(759) and Ser(789). Affinity-purified ant...
متن کاملProduction of Erythropoietin-specific polyclonal Antibodies
Background: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantification of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...
متن کاملAccurate quantitation of protein expression and site-specific phosphorylation.
A mass spectrometry-based method is described for simultaneous identification and quantitation of individual proteins and for determining changes in the levels of modifications at specific sites on individual proteins. Accurate quantitation is achieved through the use of whole-cell stable isotope labeling. This approach was applied to the detection of abundance differences of proteins present i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03344pt01